壳聚糖-甘油磷酸钠温敏凝胶及其缓释性能研究

论文编号:CL054  论文字数:11906,页数:25

摘要：本论文研究制备一种可逆温敏壳聚糖-甘油磷酸钠凝胶，并对壳聚糖-甘油磷酸钠水凝胶的温敏性能及释药行为进行考察。首先对壳聚糖的脱乙酰度以及粘均分子量进行确定，然后通过物理共混法将壳聚糖与甘油磷酸钠制成半互穿网络凝胶，研究不同体积配比,不同pH对壳聚糖-甘油磷酸钠体系凝胶化性能的影响，通过红外光谱表征壳聚糖-甘油磷酸钠凝胶；紫外分光光度计测定透光率，考察温敏凝胶相变曲线。以壳聚糖（质量浓度为2%CS）与甘油磷酸钠（浓度为56%GPS）的体积比为0.2，调整溶液pH至6.9制备凝胶，以姜黄素为模型药物，在37℃，40℃的三种PBS缓冲液中测定凝胶的药物累积释放度。实验结果表明：在37℃以上温度,壳聚糖-甘油磷酸钠凝胶可快速凝胶化（<260S），姜黄素制成载药凝胶后，姜黄素的累积释放度较姜黄素溶液显著减小。因此该温敏凝胶可用作药物的缓释载体。
关键词：壳聚糖；水凝胶类；姜黄素；缓释性
 
Abstract: This thesis is to introduce the preparation of reversibly thermosensitive chitosan-glycerophosphate hydrogel, and the investigation of its’ temperature sensitive properties and drug release behaviors. Firstly, the deacetylation degree and the viscosity average molecular weight of chitosan were determined. And then chitosan and glycerophosphate were made into semi-interpenetrating network hydrogel through the physical blending method. The effects of different mass ratio of chitosan to glycerophosphate and different pH on gelation properties of this chitosan-glycerophosphate system were studied. This chitosan-glycerophosphate hydrogel was characterized by infrared absorption spectrometry, and the phase transition curve was obtained through transmittance measured by UV spectrophotometer. Glycerol phosphate(mass concentration 56%)  and chitosan (mass concentration 2% ) in the volume ratio of 0.2, adjusting the solution pH to 6.9 to prepare thermosensitive hydrogel, curcumin as a model drug, the cumulative release rates of  curcumin were measured in three PBS buffer under 37 ℃, 40 ℃. The experimental results show that chitosan-glycerophosphate hydrogel can fast gelled (<260S) when temperature above 37 ℃, and the cumulative release of curcumin hydrogel was significantly lower than that of curcumin solution. Therefore, this temperature sensitive hydrogel can be used as drug sustained release carrier.

目  录
中文摘要 I
英文摘要 II
目录
1 绪论 1
 1.1  智能水凝胶在药物控释体系方面应用 1
 1.1.1  药物控释体系 1
 1.1.2  智能水凝胶载药体系 2
 1.2  水凝胶概述 2
 1.2.1  pH敏感水凝胶 2
 1.2.2  温度敏感水凝胶 3
 1.2.3  pH/温度双重敏感水凝胶在药物控制释放领域的应用 3
2 实验部分 4
 2.1  实验试剂和仪器 4
 2.1.1  实验试剂 4
 2.1.2  实验仪器 5
 2.2  实验方法 5
 2.2.1  壳聚糖的检测 5
 2.2.2  壳聚糖-甘油磷酸钠温敏凝胶制备方法 6
 2.2.3  凝胶制备条件的影响因素 6
 2.2.4  凝胶性能测试 7
 2.2.5  载药凝胶的制备 7
 2.2.6  载药凝胶的体外药物释放 8
3 结果与讨论 8
 3.1  壳聚糖粘均分子量的测定 8
 3.2  壳聚糖样品的脱乙酰度测定 9
 3.3  凝胶化时间的影响因素 10
 3.3.1  GPS/ CS配比对凝胶化时间和温度影响 11
 3.3.2  pH对凝胶化时间和温度影响 11
 3.3.3  温度对凝胶化时间影响 12
 3.4  透光率 12
 3.5  壳聚糖、甘油磷酸钠样品及CS-GP凝胶的红外特征检测 13
 3.6  载药凝胶的制备 15
 3.7  载药凝胶的体外药物释放 15
 3.7.1  最大吸收波长的确定 16
 3.7.2  标准曲线的测定 16
 3.7.3  药物体外累积释放度的测定 16
4 总结与展望 19
致谢 20
参考文献 21