The production of Bacillus subtilis probiotics
Abstract :Objective Optimize the production parameters of Bacillus subtilis probiotics and the conditions of Bacillus subtilis fermentation. Methods Using single factor determining Bacillus subtilis best fermentation conditions and the adoption of Bacillus subtilis processing of two dry dry way of comparison to determine the best drying methods. Results optimum fermentation conditions : temperature 37℃, pH 7.0, load 20 ml/250ml tapered bottles, speed 195 r ·min-1. Bacillus subtilis in a vacuum freeze-drying and vacuum drying agents under the conditions of the maximum number of living bacteria were up 1.1×109CFU / g and 0.7 ×109CFU/ g, the highest survival rate was 86.62% and 71.51%. Conclusion Optimal conditions for the fermentation medium ultimately counted increased by about 35.2%, the ratio of high spore formation, Optimization of cell mortality decreased over 24.7%.