Study of Immobilization of Protease On Magnetic Nanoparticles
Abstract:The Enzyme showed the advantage of rapid reaction in mild conditions ,however, there are many limitations in application of free enzyme. So in this paper，immobilization of protease was studied. Superparamagnetic nanoparticles were first immobilized with 3-aminopropyltriethoxysilane. Then glutaraldehyde was employed as a linker to the protease to the surface of nanoparticles to form Iron Oxide-protease. And then the immobilization yield of protease is measured with Bradford.
The results show that protease is immobilized successfully with maghemite nanocrystals. Under the condition of the maghemite nanocrystals Fe2O3 as a vector , the immobilization yield of protease is 40.55 percent, and under the condition of the maghemite nanocrystals Fe3O4 as the vector, the immobilization yield of protease is 46.35 percent. So, the immobilization of maghemite nanocrystals Fe3O4 and protease is better.
