The Determination Of Chlorogenic Acid Content In Xiao’er Yan
Abstract: This article is about establishing a method of quality control for assaying chlorogenic acid in Xiao’ er yan bian particle.Xiao’ er yan bian particle was analysized by HPLC.An yi li te Hypersil ODS column was used with a mobile phase consisted of mixture of acetonitrile—0.2%phosphoric acid (10:90,by volume ratio)by flowing rate of 1.0mL/min in this method.The methods of extracting chlorogenic acid by hypersound and backstreaming were equivalented.The hypersound was more convenient ,so we chose hypersound as the method to extract chlorogenic acid. Using 50% (by volume ratio) methanol as the menstruum,the time of extracting was 20 minutes.And VWD detector wavelength was 327nm.The results suggested that the linear range of chlorogenic acid was 1.016~101.6μg/mL (r= 1).The precision of sample introduction was very good by this method and chlorogenic acid was steady in 50% (by volume ratio) methanol in 12 hours . The determination reproducibility and daytime degree of precision of the method were very good.The average recovery was 98.6% (RSD=0.55%,n=6) . In Conclusion, this was an accurate, simple, rapid qualitative method with good reproducibility to assay chlorogenic acid in Xiao’ er yan bian particle.
Keywords:Xiao’ er yan bian particle;HPLC;Chlorogenic acid