Optimization of fermentation conditions for production of Rapamycin
Abstract: In this paper, the rapamycin fermentation to proceed on the slant medium, seed medium and fermentation medium to optimize the composition and concentration, and seed strains studied age, harvest time and shake-flask extraction HPLC conditions. The main experimental single-factor method to compare the impact of various factors in order to achieve the purpose of optimization. Determination of rapamycin has biological activity assay, thin layer chromatography and liquid chromatography, comparison drawn, high-performance liquid chromatography quantitative analysis can be simple rapamycin, and measured results reliable. In the medium and the optimized conditions, the output of rapamycin have been greatly improved in the optimized conditions, the production of rapamycin 140mg / L, for the further industrialization of the production of rapamycin provides the basis.
Keywords：Rapamycin；Immunosuppressant；HPLC；Optimization of fermentation conditions
