Catalytic synthesis of ethyl butyrate by immobilized lipase
Abstract: In recent years, with the rise of green chemistry and energy-saving, environmental protection, enzyme reuse has become an important topic of the biological engineering, enzyme immobilization is one of the methods to solve this problem, In no water system, immobilized enzyme system not only can alleviate deactivation function of solvent for enzyme, but also can scatter more evenly in the system, which makes it easy to handle. This experiment with towel for the carrier, with glutaraldehyde fixation lipase on adsorption crosslinking and use in the immobilized lipase studied.it butyric acid catalytic synthesis of ethanol and positive reaction. Study the immobilized enzyme process quality, the carrier, the reaction system load time, substrate concentration and mole ratio, moisture content, surfactants, storage and temperature stability of catalytic ethyl butyrate reaction and determine the influence law of the optimal conditions. At 0.6 g carrier fixed lipase 0.1 g / 10ml advisable, in the reaction system in ethanol and butyric acid optimal ratio of 1:2 for the optimal water free enzymes, surfactants, 0.5% of enzyme activity in the ascension Tween60 most obvious effect, in addition, in time, stability studies on substrates and product trend is also discussed.
