摘 要：红曲色素是一种安全、天然的食品添加剂，具有色价高、毒性低、稳定性好、对蛋白质着色强等优点，被广泛应用于食品行业中。本研究是通过对实验室保藏的一株红曲霉进行紫外诱变，从而获得了一株红曲色素产量较高的突变菌株，其发酵色价达到264.00U/g，较出发菌株提高了46.5%。在对色谱的扫描中发现该红曲红色素的最大吸收波长为500nm。同时对该突变菌株的发酵条件进行了优化，结果表明接种量为1.5ml，发酵底物为玉米粉，发酵温度为30℃时，色价最高，达到269.00U/g。并对发酵产物中红曲色素提取条件进行了研究，结果显示乙醇浓度为70%，提取时间为60min时提取效果最佳。
关键词：红曲红色素；紫外诱变； 发酵条件；提取条件
The breeding of Monascus for producting red pigment
Abstract: Monascus pigment is a safe, natural food additives，and it is widely used in food industry. In order to screening the mutants with higher activities,the mutation breedi- ng of the monascus was carried out by ultraviolet mutagenesis, the mutant strains’s color value is 264.00U/g.Then study the ferment condition of the mutant strain. The results suggested that the fermentable substrates being corn meal and the fermentation temperature being 30℃ are the optimal condition.In this fermentation condition,the color value reach 269.00U/g .Simultaneously, choose the optimum extration process of monascus red pigment.The results suggested that concentration of ethanol being 70% and the extraction time being 60min are the optimal extract condition.
Keywords：Monascus red pigment；Ulltraviolet mutagenesis；Fermentation condition；extration condition
