Analysis and evaluation of Polysaccharides and Triterpenes Contents of Fruit-body of strains of Ganoderma lucidum and Studies on protoplast fusion technique of Ganoderma lucidium
Abstract: Polysaccharides and triterpenes contents of fruit-body of twelve strains of Ganoderma lucidum were determined in order to breed special strains for extraction and processing. The result showed that the polysaccharides contents of theirs were different. Among which, G .capense, Japanese G .lucidum, G .lucidum XIANZHI, G .lucidum JINGDA and G .applanatum were higher in polysaccharide contents. While G .lucidum JINGDA, G .lucidum05, G .lucidum HUIZHOU, G .lucidum XINZHOU, G .applanatum, G .tsugae and Japanese G .lucidum were higher in triterpenes contents. There was no distinct correlation between polysaccharides and triterpenes contents of fruit-body of Ganoderma lucidum. These results provided the theoretical basis for the select and breed of special strains for processing, which Ganoderma lucidum polysaccharides and triterpenes contents are both higher.We use G .lucidum05 and G .lucidum06 conducted protoplast fusion test，received a total of 187 new strain, and there are 33 strain have antagonistic response with parents, These results provided the basis of a laboratory experiment for the select and breed of special strains for processing, which Ganoderma lucidum polysaccharides and triterpenes contents are both higher.
