The Using the squid soft bone meal preparation process of chitin exists
Abstract: Chitin is a kind of natural linear polysaccharide polymer made from the N-acetyl-D-glucosamine by β-1, 4 glycosidic bond [1]. This study and the process conditions of squid cartilage removal of protein and salt removal were optimized. By a series of single-factor tests and orthogonal trials.the concentration of sodium hydroxide, sodium hydroxide liquid ratio, time, temperature to remove protein from squid cartilage, but also to explore the frequency, concentration of hydrochloric acid, hydrochloric acid liquid ratio, time, temperature on the removal of inorganic salt squid cartilage. the products and the chitosan made from them were analyzed with infrared spectroscopy. The results show that the optimum conditions for the removal of proteins as follows: the sodium hydroxide concentration is 10%,the liquid ratio is 1:10 , the temperature is 60 ℃ and extraction time is 30 Min. The optimum conditions for salt removal is showed : 8% concentration of the hydrochloric acid, 1:14 of liquid ratio, 60 ℃ of the temperature and 40 Min of the extraction time. The infrared spectra show that the product prepared by this method is chitin and has the high purity ,and on the same preparation process conditions of chitosan, the chitosan with ultrasound-assisted preparation of chitin is obtained a higher purity of chitosan and a higher degree of substitution.
Keywords：chitin; wave; removal of protein; salt removal; infrared spectrum analysis
