Abstract: This article says the way of screening the high-concentration butanol resistant strain from the soil in nature.In the industrial production, when the concentration of n-butanol reached 11g/l, because of the inhibition of n-butanol, 50% of the strain will stop the growth. Screening of the high-concentration butanol resistant strain through gradient plate method, and then separated and purificated the butanol resistant strain. Butanol resistant strain as a result of solid medium in the growth cycle is longer, so it is necessary to carry out a series of culture in the liquid medium to determine the reason is the growth cycle is longer or the volatile of n-butanol in the solid medium. Finally, screening of the butanol resistant strain to be resistant to the concetration of 60g/l. Meanwhile, the growth curves of the strain in the liquid medium with and without n-butanol were draw, and do the oxygen resistant detection and some simple strain identification, more or less determined the butanol resistant strain is Bacillus sp. Ultimately, do the further slope preservation, and kept the butanol resistant strain in the refrigerator.
Keywords: screen; n-butanol; the growth curve; strain identification
