Abstract: The bacterial cellulose is widely appllied in food, medicine, textile and chemical industry as a new biosynthetic materia1. This article use some fruit and bagging vinegar brought from market in Xiaoheshan and soil collected in Zhejiang University of Science and Technology as screening material, after enrichment culture and repeated screening by diluting and spreading method, strain BV02 with stable productivity from vinegar was obtained. Through static culture, it can form insoluble gel-like cellulose in the liquid, The main component of gel-like cellulose by BV02 was confirmed to be cellulose. Investigating the fermentation conditions, the results showed that the static fermentation condition of BV02 was 10% of inoculum size, 24h of seed age, fermentation temperature 30℃, optimal initial pH 6.0 and culture time 7 days.