Abstract: This study, which is on the basis of early placktt-barman test results, us the design on the response surface temperature (X1), culture time (X2), the initial pH (X3)to make the three affected β-carotene yield of the main factors fitting optimization. and the results showed that: When temperature is 27.4 ℃, fermentation time is 154 h and the best initial pH is 6.7, β-carotene actual output was 796.25 ± 7mg / L. In this best fermentation conditions, we study the impact of the surfactant and oxygen carrier on the fermentation of Blakeslea trispora. Result shows that, Triton X-100, Span-20, Tween-80, these surfactant effect β-carotene and biomass both the larger, and 0.1% Triton X-100 effects most .Adding oxygen carrier can effectively improve the dissolved oxygen supply in the system of fermentation, and also increase the output of β-carotene. when add 1 percent of n-12,it reaches the highest output of β-carotene and mycelia mass. And the highest output is 923.54 mg / L, biomass to 56.12 g / L.
Key words: β-carotene; Blakeslea trispora; Surfactant; Oxygen carrier
