茯苓多糖的提取及其羟丙基化研究

论文编号:SW188  论文字数:8548,页数:21

 摘要：在超声波辅助下，采用稀碱提取茯苓多糖，探讨了提取工艺，提高了多糖提取率。将碱溶性茯苓多糖进行羟丙基化得到的水溶性羟丙基茯苓多糖，用红外光谱对其进行了结构表征。通过对胆酸结合能力测试实验可知，茯苓多糖羟丙基化后能提高多糖对胆酸的结合能力。

关键词：茯苓多糖；提取；超声波；羟丙基化；胆酸结合能力

Abstract: Alkali-soluble parchymaran was extracted by using dilute alkali (0.5mol/l NaOH) solution as a solvent with the assistance of ultrasonic and the extraction condition was examined. 1.2-epoxypropane-mediated hydroxypropylation was applied to parchymaran, and the physicochemical properties of the resulting derivatives were investigated by using FT-IR and bile acid binding activity. The hydroxypropylated derivative showed higher bile acid binding activity than native glucan .
Keywords:Polysaccharides; Extraction; Ultrasonic wave; Hydroxypropylation
Acid binding capacity.

目录
中文摘要…………………………………………………………………………Ⅰ
英文摘要…………………………………………………………………………Ⅱ
目录………………………………………………………………………………Ⅲ  
绪论……………………………………………………………………………1
1.1茯苓多糖的认识…………………………………………………………1
 1.2茯苓多糖提取及修饰的研究进展………………………………………1
 1.3茯苓多糖衍生物…………………………………………………………2
 1.4实验构想…………………………………………………………………2
2. 实验部分…………………………………………………………………….3
  2.1  材料与试剂……………………………………………………………3
   2.2 主要仪器设备………………………………………………………….3
 2.3 实验步骤……………………………………………………………….4
 2.3.1碱溶性茯苓多糖的提取及纯化………………………………….4
 2.3.2 多糖含量的测定原理及方法……………………………………6
 2.3.3 茯苓多糖样品含量测定…………………………………………6
 2.3.4羟丙基衍生物的制备…………………………………………….6
 2.3.5 茯苓多糖的红外光谱测定………………………………………7
 2.3.6 胆酸结合能力的测定……………………………………………7
 3. 结果与讨论………………………………………………………………… 8
   3.1波长的选择……………………………………………………………  8
   3.2标准曲线的制作…………………………………………………………8
 3.3 茯苓多糖的提取工艺探讨…………………………………………… .8
 3.3.1 料液比对茯苓多糖提取率的影响………………………………9
 3.3.2 碱浓度对茯苓多糖提取率的影响………………………………10
 3.3.3 超声时间对茯苓多糖提取率的影响……………………………11
 3.4红外光谱分析……………………………………………………………12
3.5 胆酸结合能力评价………………………………………………………13
4.结论与展望……………………………………………………………………15
 4.1结论………………………………………………………………………15
 4.2 展望…………………………………………………………………… 15
致 谢  ………………………………………………………………………… 16
参考文献…………………………………………………………………………17