对乙酰氨基酚的含量测定及有关物质检查

论文编号:ZY009    论文字数:7563,页数:21

 摘要：本论文建立对乙酰氨基酚含量测定的方法。采用高效液相色谱法和紫外分光光度法。高效液相色谱法以C18为色谱柱，以甲醇-水 (体积比70∶30)为流动相，流速为1.0 mL／min，检测波长为250 nm。对乙酰氨基酚在5～30 μg／mL浓度范围内呈现良好的线性关系(r=0.9997)，回收率为99.6％（RSD=1.9％)。在该色谱条件下对对乙酰氨基酚的杂质对氨基酚进行检查，对氨基酚线性范围0.1 μg/mL ～1.2 μg/mL。紫外分光光度法测定对乙酰氨基酚含量，结果表明，对乙酰氨基酚在2～10 ug／mL范围内线性关系良好，平均回收率为99.9％(RSD=1.1％)。我们建立的两种含量方法操作简便，灵敏度高，重复性好，测定结果准确，可用于对乙酰氨基酚的含量测定。
 关键词：  对乙酰氨基酚；对氨基酚；高效液相色谱法；紫外分光光度法

Abstract : The purpose of this article was to establish methods for content determination of acetaminophen. We used High-performance liquid chromatography and ultraviolet spectrophotometry. Chromatographic conditions were C18 column and methanol-water (volume ratio 70:30) as the mobile phase. The flow rate was 1.0 mL / min and the detection wavelength was 250nm. In the range of 5～30 μg／mL,  acetaminophen’s concentration showed a good linear relationship (r = 0.9997). The recovery  ratio was 99.6% (RSD = 1.9%). The p-aminophenol  was detected under the same conditions and its linear range was 0.1μg/mL~1.2μg/mL. UV spectrophotometry was used to determine the content of acetaminophen. In the range of 1~10ug/mL, the concentration showed a good linear relationship and the average recovery ratio was 99.9%(RSD = 1.1%). The two methods were simple,  sensitive,  reproducible and accurate, which can be used in the content determination of acetaminophen.

Key words： Acetaminophen；p-Aminophenol ；HPLC； UV spectrophotometry
目    录

1.  绪论 …………………………………………………………    1
 1.1  对乙酰氨基酚…………………………………………    1
 1.2  选题背景与意义………………………………………    1
 1.3  含量测定方法…………………………………………    2
2.  实验部分 ……………………………………………………    4
 2.1  高效液相色谱法………………………………………    4   
 2.2  紫外分光光度法………………………………………    6
3.  结果与讨论 …………………………………………………    7
 3.1  结果……………………………………………………    7
 3.2  讨论……………………………………………………    12
4.  总结与展望 …………………………………………………    13
致谢 ………………………………………………………………    14
参考文献 …………………………………………………………    15