茶皂素的纯化与分析测定

论文编号:ZY079    论文字数:7446,页数:15  附任务书，开题报告，文献综述，外文翻译   PPT

摘要：本文综述了茶皂素的性质、提取精制及油茶皂素定量方法的研究，并总结了当前茶皂素研究中最值得进一步研究的问题。对粗茶皂素的提取纯化工艺进行了研究。以质量分数为70％的粗茶皂素为原料，经2％ NaOH溶解、盐酸酸析、95％ 乙醇溶解、丙酮沉析工艺得到精制茶皂素。研究了用反相高效液相色谱法(HPLC)测定茶皂素含量的方法，以Hewlett Packard ODS Hypersil 5 微米，125×4毫米不锈钢柱作分析柱，以甲醇作流动相，流速控制在0.5mL／min，检测波长276nm。通过反相高效液相色谱法测定经过精制后的茶皂素的含量为97.6709％。
关键词：反向高效液相色谱；茶皂素；提取纯化

Abstract：This paper summarizes the development of the property, extraction and purification, and quantitative determination of tea saponin. Besides, issues needing further study were indicated .A simple purification method for crude tea saponin was described. The 70%of crude tea saponin was dissolved in 2% sodium hydroxide aqueous solution and precipitated by adding 20% hydrochloric acid. The deposit was dissolved with 95%ethanol, and the acetone was added to the ethanol solution to make the tea saponin to be precipitated. A reverse phase high performance liquid chromatography (HPLC) method for determination of tea saponin extracted from tea-seed pancake with Soxhlet was studied. With the column Hewlett Packard ODS Hypersil (5µm 125mm*4mm)and mobile phase methanol at flow-rate of 0.5 ml/min. The recovery rate of tea saponin was 97.6709%
Key words：reverse-phase high perform ance liquid chromat0graphy;tea saponin; purification

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1  概述 1
 1.2 茶皂素的理化特性 1 
 1.3 茶皂素的实际用途 1
 1.3.1 茶皂素的溶血作用及鱼毒作用 1
 1.3.2 茶皂素在医药上的应用 2
 1.3.3 工业上的应用 2-3
 1.3.4 农业上的应用 3
2. 实验部分 4
 2.1 试剂与仪器 4
 2.1.1 实验材料 4
 2.1.2 主要试剂 4
 2.1.3  主要仪器设备 4 
 2.2 实验步骤 5
 2.2.1实验中溶液的准备 5
 2.2.2含量80%粗提取物的精制 5
 2.2.3供试品溶液的配制 5-6
 2.2.4操作条件 6-7 
 2.2.5含量的测定 7-10
3. 结果与讨论 10
 3.1 精制过程中PH和温度的控制    10
 3.2 讨论 10-12
4．总结与展望 12
 4.1 实验总结 12
 4.2 展望 12-13
致谢 14
参考文献 15