Abstract:Nowadays，the hybridity of catalysis of enzyme is the focus areas of catalytic current. It is a capability which can catalyst two or more different types of chemical reactions by the same catalytic core. The expedition of the hybridity of catalysis of enzyme，provides a new way of thinking for father studying of the relationship between structure and function of the enzyme and developing the new functional of enzymes. By screening several enzymes which have the ability of catalytic knoevenagel reaction，we find the PPA has a very good catalysis to this reaction at the first time. In the reaction，we find that Lipase can not only catalytic the reaction，but also execute the original reaction of transesterification. Through the study of this reaction，we find that Amylase Have good catalytic to this reaction. The experiment not only expanded the field of enzyme catalysis，but also have a certain significance to the evolution of enzyme.
