Object：Investigate the extraction of polysaccharides cordate houttuynia Preparation，its preparation has commonly used ultrasonic extraction method, alkali extraction, acid extraction method.
Ways：To yield polysaccharide, polysaccharide content as an index component, orthogonal test of the above three methods of extraction of polysaccharides cordate houttuynia optimization process. The results showed that the best cordate houttuynia polysaccharide extraction process as follows: liquid-solid ratio (V / m, mL / g, the same below) 30:1, 80 ℃ water bath extracted 3 times 2h, extraction rate of polysaccharides 3.5%. In addition to protein, gel chromatography on a column of pure polysaccharide with WatersSep - pak C18 solid phase extraction column pre-separation to efficient sugar Waters carbohydrate column as the stationary phase, V (acetonitrile): V ( water) = 70:30 as mobile phase separation, evaporative light scattering detector instrument to detect, to be composed of polysaccharide cordate houttuynia m (xylose): m (fructose): m (arabinose): m (galactose) = 2. 209:1. 587:1. 000:2. 092..
Conclusion: from the polysaccharide yield and content can be drawn in three ways ultrasonic extraction method as the best technology, the method is simple, accurate, high sensitivity, suitable for the extraction of polysaccharides cordate houttuynia.
