海带中褐藻糖胶提取工艺研究

论文编号:SP041  论文字数:9223,页数:21  附任务书,开题报告

摘  要：海带含有多种生物活性成分，如海带酸、褐藻酸、甘露醇和褐藻糖胶等，其中褐藻糖胶具有降血脂、抗血凝、抑制肿瘤细胞生长、调节免疫力、抗辐射等作用。鉴于褐藻糖胶的诸多保健功能和我国有丰富的海带资源，开展对海带资源的深加工和综合利用、充分发挥和利用褐藻糖胶这以天然多糖资源，对提高海带附加值、发展我国海带资源加工有深远的意义。本文采用酸提法从海带中提取褐藻糖胶，采用乙醇分级沉淀法进行纯化。主要探讨了提取时间、温度、pH、料液比等因素对提取率的影响。结果表明，在提取温度70 ℃，pH2.0，液料比为30，提取4h时，提取率最高，达到7.3%。
关键词：海带；褐藻糖胶；酸提法；沉淀法

Abstract: The kelp contains a variety of biological active ingredients, such as kelp acid,brown alga acid,mannitol and fucoidan,among which fucoidan has hypolipidemic effect on anti-blood congealing,inhibits the growth of tumor cells to regulate immune function,anti-radiation effects.
In view of the fact that fucoidan has many health functions,and China has abundant seaweed re - sources,therefore,the development to the kelp resources,intensive processing and the comprehen- sive utilization,the full display and the use fucoidan which is natural polysaccharide resource,to enhance the kelp added value and to develop our country kelp resources processing has the pro- found significance.This paper uses the sour formulation to extract the fucoidan from the kelp,uses the ethyl alcohol graduation precipitation method to carry on the purification. Focuses on the extr- action time,the temperature,pH,solid to liquid ratio on the extraction rate. The result indicated that in the extraction temperature 70℃,pH 2.0,70% ethyl alcohol density,extraction 4 h,the extraction rate is 7.3%.
Key words: kelp; fucoidan;acid distill;precipitation

目  录
1. 前  言 4
1.1 选题背景 4
1.2 褐藻糖胶的综述 4
1.2.1  褐藻概况 4
1.2.2  褐藻糖胶概况 4
1.2.3  褐藻糖胶的生物活性功能 5
1.2.4  褐藻糖胶国内外研究现状 6
1.3  研究方法 8
1.4  研究目的和意义 8
1.5  预期结果 8
2.材料与实验方法 9
2.1  材料与仪器 9
2.1.1  原料与试剂 9
2.1.2  仪器 9
2.2  实验方法 9
2.2.1  褐藻糖胶的提取 9
2.2.2  褐藻糖胶的纯化 10
2.2.3  褐藻糖胶的测定 10
2.3  不同实验条件对海带中褐藻糖胶提取影响 10
2.3.1  pH单因素实验 10
2.3.2  温度单因素实验 10
2.3.3  时间单因素实验 11
2.3.4  液料比单因素实验 11
3. 结果与分析 12
3.1  pH单因素实验结果与分析 12
3.2  温度单因素实验结果与分析 13
3.3  时间单因素实验结果与分析 14
3.4  液料比单因素实验结果与分析 15
3.5  结论 16
4. 褐藻糖胶的研究前景 17
参考文献 18