粗毛栓菌MnP的分离与纯化

论文编号:SP046  论文字数:4542,页数:07,附任务书,开题报告,中期检查,答辩记录

粗毛栓菌MnP的分离与纯化
   
摘要：粗酶液来自麦草粉固态培养物，经超滤浓缩、盐析、透析、Q- Sepharose fast flow阴离子交换层析后获得具有MnP活性的组分。
关键词：粗毛栓菌   MnP  离子交换层析

Isolation and Purification of MnP in Trametes gallica Fr.

Abstract：The crude enzyme from wheat straw solid culture of  Trametes gallica Fr. was ultra-filtrated, salted-out, dialyzed and applied to Sepharose-Q Fast Flow ion exchange column. A component with manganese peroxidase activity was obtained.
Key words: Trametes gallica Fr.,Manganese Peroxidase, ion exchange chromatography.

 
目 录
 
摘要……………………………………………………………………………………………1
关键词…………………………………………………………………………………………1
Abstract………………………………………………………………………………………1
Keyword………………………………………………………………………………………1
引言………………………………………………………………………………………1
1 材料与方法…………………………………………………………………………………2
1.1 主要仪器与试剂…………………………………………………………………………2
1.2 菌株及培养基·……………………………………………………………………………2

1.2.1  菌株……………………………………………………………………………………2
1.2.2  培养基…………………………………………………………………………………2
1.2.3  原酶液的来源…………………………………………………………………………2
1.3  锰过氧化物酶的分离与纯化步骤………………………………………………………2
1.3.1  粗酶液的制备…………………………………………………………………………2
1.3.2  硫酸铵盐析……………………………………………………………………………3
1.3.3  透析……………………………………………………………………………………3
1.3.4  Q-Sepharose Fast Flow 阴离子交换层析……………………………………………3
1.3.5  MnP酶活力的测定·…………………………………………………………………3
1.3.6  蛋白质含量测定………………………………………………………………………4
1.3.7  浓缩……………………………………………………………………………………4
2 结果与分析…………………………………………………………………………………4
2.1  MnP的柱层析纯化结果………………………………………………………………4
2.2  蛋白含量测定结果……………………………………………………………………5 
3  讨论………………………………………………………………………………………5
参考文献………………………………………………………………………………………6
致谢………………………………………………………………………………………6