Preliminary Isolation and purification
of xylanase in Trametes gallica Fr.
Abstract The crude enzyme from wheat-straw culture of Trametes gallica Fr. was ultrafiltrated, salted-out ,dialyzed and applied to Sephadex G-150 molecular sieving column.A component with xylanase activity was eventully obtained by native polyacrylamide gel electrophoresis.
Key words Trametes gallica,xylanase,Sephadex G-150 molecular sieving,native-PAGE