粗毛栓菌木质素过氧化物酶的分离与纯化

论文编号:SP057  论文字数:4121,页数:07,附任务书,开题报告,中期检查,答辩记录

粗毛栓菌木质素过氧化物酶的分离与纯化
摘要：将粗毛栓菌接种在含有麦草粉的培养基中培养60d，浸提得粗酶液，经超滤浓缩、盐析和透析，将透析液上样于Q-Sepharose F. F.层析柱，获得具有木质素过氧化物酶活性的组分。
关键词：粗毛栓菌，木质素过氧化物酶，离子交换柱层析

Separation and Purification of Lignin Peroxidase in Trametes gallica
Abstract： Trametes gallica was cultivated in wheat straw powder for 60 days. The crude enzyme by ultrafiltrating, salting and dialysis, was applied to Q-Sepharose FF ion exchage column. The effluent with Lignin peroxidase acticity was obtained.
Key words：  Trametes gallica,  lignin peroxidase,  ion exchage chromatography

 目  录

摘要……………………………………………………………………………………………1
关键词…………………………………………………………………………………………1
Abstract………………………………………………………………………………………1
Key words…………………………………………………………………………………… 1
引言……………………………………………………………………………………………1
1材料与方法…………………………………………………………………………………1
1.1菌株………………………………………………………………………………………1
1.2主要仪器及试剂…………………………………………………………………………2
1.3培养基……………………………………………………………………………………2
1.4样品的制备………………………………………………………………………………3
1.4.1粗酶液的制备…………………………………………………………………………3
1.4.2酶的超滤浓缩…………………………………………………………………………3
1.5硫酸铵沉淀………………………………………………………………………………3
1.6离子交换层析……………………………………………………………………………3
2结果…………………………………………………………………………………………4
2.1酶活测定…………………………………………………………………………………4
2.2洗脱曲线…………………………………………………………………………………4
3讨论…………………………………………………………………………………………5
参考文献………………………………………………………………………………………5
致谢……………………………………………………………………………………………6