关键词：枯草芽孢杆菌；芽孢形成；培养基；培养条件
Abstract: Sterilization of aseptic filling equipment, if poor, drinks in aseptic filling process, the microbes may be breeding. So aseptic filling equipment in the factory needs to aseptic validation. Bacillus subtilis is a bacteria that can not easily be killed, often used as test bacteria. The thesis optimized medium components of Bacillus subtilis and culture conditions, For the aseptic filling of the safety evaluation done for it. Through single factor test, optimize the most suitable medium composition and culture conditions. The results showed that Bacillus subtilis optimum medium: starch 5g/L, beef extract 5g/L, NaCl 3g/L, K2HPO4 3g/L, MnSO4.H2O 2.4mmol. And medium pH adjusted to 7.0, in 250mL flask with 10% of the medium ,at 37 ℃ , shaking speed is 150r/min. Under these conditions, viable count, spore count and spore rate to the highest. This study used the plate count and turbidity was studied strains of Bacillus subtilis ATCC9372 growth curve, the results show that the optimal vaccination age of 6 hours.
