Abstract：Anthocyanins have effective anti-aging, anti-cardiovascular, anti-cancer functions, and also play a role in anti-radiation, anti-fatigue and improving memory, especially in the respect of clearing harmful free radical of the body. They have showed extremely superior bioactivity and safety. However, our bodies can’t produce any anthocyanins, we should only take them from food or other supplements. It has been reported that extraction and preparation of high purity anthocyanins has wide application prospects.
This work studied the extraction of anthocyanins from dioscorea batata decne. The effects of solvent concentration, ratio of sample to solvent, extraction temperature and extraction time on the yield of anthocyanins were studied. Based on the data of single-factor experiment, the optimum conditions were evaluated further applying the orthogonal testing method with intuitive analysis and variance analysis. The preliminary majorized extraction conditions are as follows: extraction solvent of 60% (volume fraction) alcohol solution, ratio of sample to solvent 1:12 (g/mL), extraction temperature 60 ℃, extraction time 13 h. Under these conditions, the yield of anthocyanins is 3.90%.
