Abstract:Bacillus subtilis showed strongly antibacterial activity against Aspergillus niger.A crude antibacterial preparation(CAP) was obtained by adding ammonium sulphate to supernatant fluid of B.subtilis to a finial saturation of 60%.CAP was purified by dialysision.CAP is thermostable;its active pH range is wide,from 4.0 to more than 10.0,but it is sensitive to high pH;it is sensitive to UV rays.
Key words:B.subtilis antibacterial peptite separation and purification antibacterial character