一种微生物源性抗菌蛋白的提取

论文编号:SP109  论文字数:6241,页数:16

摘要：枯草芽孢杆菌对黑曲霉有强烈的抑制作用。于培养液上清中加入60%饱和度的硫酸铵沉淀可得蛋白粗提液。该蛋白粗提液经透析脱盐后得到初步纯化。该蛋白耐高温；它在从4到10的很广的pH范围内能保持活性，但对高pH值敏感；对紫外线不敏感。
关键词：枯草芽孢杆菌；抗菌蛋白；分离纯化；抗菌特性

Abstract:Bacillus subtilis showed strongly antibacterial activity against Aspergillus niger.A crude antibacterial preparation(CAP) was obtained by adding ammonium sulphate to supernatant fluid of B.subtilis to a finial saturation of 60%.CAP was purified by dialysision.CAP is thermostable;its active pH range is wide,from 4.0 to more than 10.0,but it is sensitive to high pH;it is sensitive to UV rays.
Key words:B.subtilis  antibacterial peptite  separation and purification  antibacterial character

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目录
中文摘要…………………………………………………………………. Ⅰ
英文摘要…………………………………………………………………...Ⅱ
目录…………………………………………………………………………Ⅲ
1.绪论………………………………………………………………………..1
2.实验部分 2
2.1材料和方法…………………………………………………………..2
2.1.1材料…………………………………………………………….2
2.1.2方法…………………………………………………………….2
3.结果与讨论.................................................5
3.1结果...................................................5
3.1.1菌株观察..........................................5
3.1.2菌株发酵..........................................6
3.1.3抗菌蛋白的分离纯化................................6
      3.1.4抗菌蛋白理化性质的研究……………………………………..7
3.2讨论……………………………………………………………………9
3.2.1发酵……………………………………………………………..10
3.2.2抗菌蛋白的分离纯化
4.总结与展望…………………………………………………………………….6
4.1总结……………………………………………………………………….6
4.2展望......................................................6
致谢...........................................................8
参考文献.......................................................9