利用微卫星引物Bd43A分析桔小实蝇的多态性

论文编号:SP026 论文字数:8236,页数:20

摘    要
   本论文利用微卫星标记对四川、云南、广西、福建四个地区桔小实蝇种群的遗传多态性进行了分析。通过提取其基因组DNA,利用微卫星引物Bd43A进行PCR扩增,并进行聚丙烯酰胺凝胶电泳，利用Popgene软件进行数据分析。结果表明,这四个地区的种群都有较高的遗传多态性；福建和广西、四川和云南的桔小实蝇种群具有很近的亲缘关系。
关键字  桔小实蝇  遗传多态性  微卫星

netic Polymorphism Analysis with Microsatellite Marker Bd43A of Bactrocera dorsalis
Abstract:    By using microsatellite markers, the genetic polymorphism of 4 Bactrocera dorsalis populations collected from provinces of Sichuan, Yunnan, Guangxi and Fujian were analyzed. After extracting the genome DNA, microsatellite primers Bd43A were amplified, and electrophoresed by polyacrylamide gel. Analysed by software Popgene, it was shown that the four Bactrocera dorsalis populations had high genetic polymorphism. The relationships between Fujian and Guangxi, Sichuan and Yunnan were near.
Key words：Bactrocera dorsalis;genetic polymorphism;microsatellite

目        录
1  前言 1
2  材料与方法 2
2.1  材料 2
2.1.1  样品 2
2.1.2  主要仪器设备和药品 2
2.2  方法 3
2.2.1  桔小实蝇成虫采样 4
2.2.2  试剂盒提取桔小实蝇总DNA 4
2.2.3  PCR扩增 4
2.2.3.1  PCR反应引物 4
2.2.3.2  PCR反应体系 5
2.2.3.3  PCR反应程序 5
2.2.3.4  PCR产物的聚丙烯酰胺凝胶电泳及分析 5
2.3  数据记录和分析 6
3  结果与分析 6
3.1  微卫星引物在桔小实蝇种群中的扩增结果 6
3.2  桔小实蝇地理种群的遗传多样性 8
3.3  桔小实蝇地理种群的分化 9
3.4  桔小实蝇地理种群的聚类 10
4  讨论 10
参考文献 12
英文摘要 13
致谢 14
成绩评定表及答辩记录 15