Optimization of Ultrasound-assisted Extraction of Limonin in Orange Nuclear by the Orthogonal Design
Abstract: In order to determine the effects of extraction, we first examined the solid-liquid ratio，ultrasonic time，ultrasonic temperature, ultrasonic power, extraction agents types and ethanol concentration in this paper, and finally determined that solid-liquid ratio, ultrasonic time and ultrasonic temperature were the most important factors. On this basis, we optimized extraction process conditions by the orthogonal design. The result showed that the optimum conditions for the extraction conditions under the assistance of the ultrasonic are as follows: ultrasonic temperature of 30℃, ultrasonic time of 30min, solid-liquid ratio of 1:70.At the same time, we also studied the separation purification, and determined the purity of limonin is 84.94%.
