Abstract: In this article the optimization experiment of the extraction of phytosterols from rapeseed crude oil was carried out and the optimal extraction condition was determined, and accordingly to be used in industrialization extracting of phytosterols. In the experiment extraction yield of phytosterol from 10mL rapeseed crude oil was taken as an index, extraction agent, extraction temperature, extraction times, solid-liquid ratio, and extraction time in the extraction process were studied, finally the experiment was optimized by orthogonal. The results showed that under the solid-liquid ratio was 5:1(V/V), the time of extraction was 45 min, the extractant volume was 50 mL，the temperature of extraction was 93℃, the extractant tim was 1, liquid ratio was 5:1; The solvent could be recycled. Obtained by orthogonal design, the better conditions were obtained by orthogonal design as follows: when extracted unsaponifiable matter, adding sodium hydroxide-ethanol 40mL, saponification at 93℃ under 60min, then 110mL ether extraction 1 time.
Keywords：Rapeseed crude oil；Phytosterols；Extraction；Orthogonal optimization
