Abstract:Peanut hulls as raw materials produced in Shandong Province. On the base of the single factor experiments, the optimum conditions for the microwave extraction of luteolin from Peanut hulls were determined using response surface methodology(RSM). The optimum microwave extraction conditions were:extraction time 8.5min, NaOH concentration 0.1mol / L, solid to liquid ratio 1:20.5. Under the optimum conditions, the extraction yield of luteolin from Peanut hulls was 3.91%.The macroporous adsorption resin was used to separate the luteolin. Different macroporous resin type and separation condition were studied, The resin of D101 was the best one for separation, the eluting speed was 1ml/min and the volume was 3BV, the purification yield was 81.03%.