Abstract: The optimization experiment of puerarin flavonoids was carried out and the optimal extraction condition was determined, and accordingly to be used in industrialization extracting of puerarin flavonoids. In the experiment Puerarin flavonoids yield was taken as an index, mass fraction of ethanol, extraction time, solid-liquid ratio, extraction temperature, solution pH, extraction times, repeatability, recovery and reuse of solvent in the extraction process were studied, finally the experiment was optimized by orthogonal. The results showed that the mass fraction of ethanol was 70%, the extraction time was 120min, the solid-liquid ratio was 1:10, the extraction temperature was 70℃, the solution pH was 5, the extraction times was 1; repeatability for extraction operation was good, recycling solvents was feasible and the solvent recovery was up to 94.3%. The better conditions were obtained by orthogonal design as follows: the mass fraction of ethanol was 50%, the extraction time was 120min, the solid-liquid ratio was 1:12, the extraction temperature was 80℃.
Keywords: Puerarin Flavonoids; Extraction process; Yield; Solvent recovery; Orthogonal design
