Abstract: At Present, study on the bioreactor system was focused on macro-dynamics. A lot of research has been done to the dynamics of immobilized enzyme, however, study on dynamics of immobilized cells remained low level. Actually, industrial production was charged with experience of the experts. For the immobilized cells has comparative advantage, which will play more and more important part in industrial production and academic research in future, a new acetic acid production process was designed in this paper. Immobilized cells were main used in the Craft. Immobilized acetic acid bacteria was prepared by calcium alginate embedding, the average diameter of immobilized cell of 5.0 mm was obtained. The best Strain expanding condition was measured : vaccination 15 rings and cultivate 43 hours. The standard curve of glucose (OD540) was measured : y=0.2117x; and the standard curve of the bacterial cell (OD) was measured: y=402.88x. growth curve of the immobilized acetic acid bacteria basis on different alcohol concentrations and the relational curve between substrate consumption (ethanol and glucose) and production (acetic acid) have been measured. As initial concentration of ethanol changes, the acid production and the bacteria growth would be impacted: acid production was effected when alcohol concentration was too high or too low. As the concentration of ethanol increasing, bacteria for the extension of demurrage will be extended. The range of suitable initial ethanol concentration was 4% to 8%, and best fermentation time were 96 hours.
Key words: Immobilized cells；dynamic model；acetic acid bacteria； the growth curve； fermentation
