Title Ultrasonic extraction the total flavonoid of epimedii
Abstrct
In this paper,using ultrasonic extraction method, using ultrasonic produce a strong vibration,high acceleration, the strong effect of the air,stirring role of the drug would speed up the dissolution of the active ingredients and spread, thereby enhancing leaching rate, reduce the extraction time, and to avoid the high temperature The impact of proposed composition, with NaOH solution for qualitative identification of the product, with high performance liquid chromatography to determine content.
The orthogonal experiment of four factors and three levels were used to select optimum extraction method,in the root of the total flavonoid of epimedii. the factors influenced the extraction efficiency were in following:Extraction time,The number of extraction,solvent volume,ethanol concentration. Eventually selected the best extraction process to join the 95 percent ethanol solution of 800 ml, two extraction, extraction two hours each time.
Keywords Herba epimedii;Total flavonoid of Herba epimedii;Orthogonal experiment