Fig. 2. Electropherogram of iridoid substances 1D4 in Plantago lanceolata L. 1, Iridoid related substance No. 1;2, catalpol; 3, aucubin; 4, iridoid related substance No. 2.MEKC conditions: uncoated silica capillary, 50 μm i.d. ¥35 cm; running buffer, 180 mmol/l SDS and 50 mmol/l borate (pH = 9.35); separation voltage, 10 kV; current,80 μA; injection, 50 mbar ¥ 5 s; wavelengths, 200 e 2 nm and 250 e 30 nm; cassette temperature, 25 ºC.
。
Fig. 3. Electropherogram of a Plantago argentea Chaix aqueous extract. 1, Iridoid related substance No. 1; 2, catalpol;3, aucubin. MEKC conditions are the same as in Fig. 2.
Table I. Aucubin and catalpol contents in Plantago species.
Sample name Aucubin Catalpol
Average Standard LCLa UCLb Average Standard LCLa UCLb
yield (%) deviation (0.05%) (0.05%) yield (%) deviation (0.05%) (0.05%)
P. altissima 0.092 0.001 0.090 0.094 0.131 0.001 0.127 0.135
P. argentea 0.273 0.004 0.267 0.278 1.809 0.031 1.767 1.852
P. coronopus < LODc < LODc
P. holosteum subsp. 0.077 0.007 0.068 0.086 < LODc
depauperata
P. holosteum subsp. < LOQd < LODc
holosteum
P. holosteum subsp. < LOQd < LODc
scopulorum
P. lagopus 0.072 0.007 0.063 0.082 0.082 0.002 0.079 0.085
P. maritima 0.027 0.003 0.021 0.034 < LODc
P. lanceolata 0.115 0.010 0.098 0.132 0.159 0.001 0.152 0.166
a Lower confidence limit.
b Upper confidence limit.
c Limit of detection.
d Limit of quantification.
The authors would like to thank to Dr. Siren Rosendal Jensen for the iridoid standards, and to Goran Srecˇnik for technical support and useful suggestions.
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