The Study on Extraction Chitin from Fly Pupa Shell
Abstract：The method of extracting chitin and preparation chitosan from fly pupa shell was studied. The extracting steps were including breaking, screening and collecting fly pupa shell, and then washing, detaching calcium, detaching protein, decolorizing, detaching acetyl and drying. At the end, the product of chitin and chitosan was got. The optimum detaching calcium condition was: the ratio of fly pupa shell and hydrochlotic acid (5%) being 1g: 10ml, immersed and stirring 4 h under normal temperature. At the same time, the optimum detaching protein was: first, the material was reacted with 2%NaOH for 4 h under normal temperature, after filtered, reacted with 10%NaOH under hot water bath. The best reagent used to decolorize was H2O2. At last, it was reacted with 40% NaOH for 4 h under 120 degree Celsius with stirring and a white chitosan with higher acetyl-detaching degree could be got.
