Bacillus subtilis Optimization Training (liquid fermentation)
Abstract : The single factor and orthogonal experiment. of Bacillus subtilis (Bacillus subtilis) and the liquid fermentation medium conditions. primal bacterium medium carbon, nitrogen ratio and the choice of adjustment to determine the elements of a reasonable ratio. Determine the best medium : 3% sucrose, 1% peptone, yeast extract 0.5% KH2PO4 0.45% (NH4) 2SO4 0.25%, 0.2% CaCO3; also a single medium fermentation conditions change, the results of the conditions through the cultivation of the best values application specific experimental operation, and eventually the temperature, pH value, inoculum load and the best combination of four factors fermentation, and this combination of certification, proved the best mix of bacterium can truly improve training purposes. Experimental evidence for the optimum conditions : temperature 30%, pH 7.0, 20ml/250ml installed capacity of tapered bottles inoculation of 5% speed agitation conditions.
Keywords：Bacillus subtilis； Medium ；Fermentation conditions；Growth.
