发酵法生产虾青素代谢过程研究

论文编号:SW076  论文字数:12666,页数:34

摘 要：主要以改变培养基中碳源、氮源、装液量进行酵母发酵法生产虾青素摇瓶发酵过程影响的实验研究，从而测定出最佳的培养基配方。葡萄糖30g/l,（NH4）2SO4 1.5g/l，酵母膏2g/l。种瓶至发酵摇瓶的接种时间为40h，装液量为50mL。根据最佳培养基配方进行对高产虾青素生产菌性质研究、追加实验。确定发酵的基本条件，为进行酵母高产虾青素菌种的筛选做准备。研究了不同培养基条件下，酵母生长情况，为进一步研究打下基础。酵母生长通过延滞期（0-40小时），对数期（40-50小时），稳定期（50-60小时），衰亡期（60小时以后）使菌体浓度变化以缓慢增长到急剧上升再到稳定增长的过程。

Zymotechnics production astaxanthin metabolism process study
Abstract: In this paper, culture medium carbon source, nitrogen source and liquid volume were researched to find its influence on yeast production of astaxanthin. The best culture medium formula was as follows: glucose30g/l, （NH4）2SO4 1.5g/l，yeast paste 2g/l, Rocker vaccination time 40h, liquid volume 50mL. According to best culture medium formula conducts the high production of astaxanthin. Yeast growth situation were also studied. Yeast growth includes lag phase( 0-40 hours), logarithmic phase(40-50hours), stabilization period(50-60hours) and decline phase(60hours later). Causes the mycelium density change from grows slowly to rises suddenly, again arrives the stable growth the process.
关键词：虾青素，酵母菌，培养基

Keywords：Astaxanthin; Phaffia rhodozyma; Culture medium

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1 虾青素的研究发展 1
 1.2 虾青素的生产 2
 1.3虾青素生物功效及疾病的关系 3
 1.4现状及前景展望 5
 1.5本章小结 5
2. 实验部分 6
 2.1 实验材料 6
     2.2 实验方法 6
     2.3 本章小结 6
3. 结果与分析 7
 3.1 不同培养基氮源对酵母生长的影响 7
     3.2 不同培养基碳源对酵母生长的影响 8
     3.3 培养基中碳源，氮源含量的确定 9
     3.4 本章小结 18
4．总结与展望 19
致谢 20
参考文献 21