Agrocybe cylindracea fermentation conditions of optimization
Abstract:This research mainly for Agrocybe cylindracea fermentation medium and fermentation conditions optimization, exopolysaccharide extraction and the optimal fermentation conditions.
This study researchs different carbon sources, nitrogen sources, inorganic salt concentration influences the dry weight of mycelium Agrocybe and Exopolysaccharide Yield by using shake flask fermentation. and orthogonal test was exerted to study Agrocybe cylindracea’s fermentation of optimal condition.And fermentation of optimal condition is: corn meal 2%,dusty yeast 0.3%, KH2PO4 0.2%, MgSO4 0.05%.While when the carbon source, nitrogen concentration increased further, the dry weight of mycelia and exopolysaccharide production both decrease, indicating that high concentrations of carbon and nitrogen sources on the Agrocybe inhibit fermentation. In addition,we can see that Agrocybe Exopolysaccharide production and mycelial growth into a positive relationship.
At the same time,we have a test of liquid medium volume about 300mL shake flask。We find that the volume of liquid 80mL it have maximum dry weight of mycelium and production of exopolysaccharide.
Keywords:Agrocybe; orthogonal test; submerged fermentation; Optimization; loaded liquid
目 录
中文摘要 Ⅰ
英文摘要 Ⅱ
目录 Ⅲ
1. 绪论 1
1.1 食用菌深层发酵研究概况 1
1.2 真菌多糖的研究概况 3
1.3 茶树菇概述 7
2.实验部分 9
2.1 实验材料和设备 9
2.2 实验方法 10
3 实验结果与讨论 12
3.1 碳源对茶树菇菌丝体干重及胞外粗多糖产量的影响 12
3.2 氮源对茶树菇菌丝体干重及胞外粗多糖产量的影响 13
3.3 两种无机盐浓度对茶树菇菌丝体干重及胞外粗多糖产量的影响 13
3.4 正交试验优化培养基组成 14
3.5 摇瓶装液量对茶树菇菌丝体干重及胞外多糖产量的影响 16
3.6 小结 17
4 总结与展望 17
致 谢 19
参考文献 20
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