Abstract: Mildiomycin cytosine analogue (MIL-C), a new nucleoside antibiotic, showed remarkable biological activity against powdery mildews of many plants with low toxicity. This dissertation will pay attention to the process of separating MIL-C from fermentation broth. MIL-C is expected to be separated by ion exchange resins. A weakly cationic exchange resin DK110 was selected from 8 different resins, which showed high static exchange capability and good dynamic character. The effect of pH, temperature, inlet concentration, flow rate to the process of adsorption was studied in this experiment. The temperature for adsorption process was selected as 25℃ because the exchange capability is not affected by the temperament and the inlet concentration. The optimized adsorption was obtained at pH 7.0, flow rate 2BV/h. The elution conditions were determined as follows: 2% NH3·H20 solution, elution flow rate 2BV/h. An effective technology to decolorize the fermentation broth was investigated in this thesis. D261 resin, a kind of anionic exchange resin, was selected and the discoloring conditions were optimized as follows: pH7.0, discoloring time 120 min. Under these conditions, the recovery efficiency achieved 84.71%.
Key words: Mildiomycin cytosine analogue; Ion exchange; DK110 resin
