Immobilized Cell Production Of Acetic Acid Bacteria Optimization
Abstract: This paper is based on alginate as a carrier of fixed acetic acid fermentation ethanol production of acetic acid. Study of the immobilized cells Preparation, preparation of particle radius and the size. Also, the ring vaccination, seed cultivation time for the optimization. The most important thing is the alcohol of different initial concentrations of acid production, cell number, cell growth cycle, glucose consumption, the effects of ethanol consumption. Finally, we get the following conclusions : (1) a fixed cell average particle diameter of 5.0mm, particle size of 65.42mm3; (2) optimize the number of vaccines in 15 Central, Training time for between 36-48 hours, the number of bacteria broth up; (3) The concentration of glucose and glucose OD value of the conversion formula : y = 4.7237x, unit of mg/ml; (4) the number of bacteria cells OD value of the correlation : y = 402.88x, 107 units/ml; (5) the initial alcohol concentration of acetic acid production : in the alcohol concentration. 5% alcohol concentration of acetic acid fermentation broth highest conversion rate, the best time to train 96 hours; (6) the initial alcohol concentration of cell number : The impact of different initial concentrations of alcohol affects cell growth, The higher the alcohol concentration in the cell growth plays an extended period of growth delay; (7) the initial alcohol concentration of glucose consumption : The impact of the higher ethanol content of the glucose consumption is smaller; (8) the initial alcohol concentration of ethanol consumption : The impact of the 72-hour time point, ethanol consumption has been completed, acetate in 72 -96 hours of the continued generation, Acetaldehyde is not with the consumption of alcohol finished immediately into acetic acid resulting from the lagging response
Keywords：Immobilized；Ethanol；Acetic acid bacteria；Calcium magnesium acetate
