Abstract：Chitin is a very prosperous and important natural macromolecule. It is also a reproducible new macromolecule material and has no pollution to environment. Chitinase was paid attention in the research and development of biological control and medicine in recent years. Chitinase is mainly produced by microbe, whose degradation products have a wide application. The purpose of this study is purification and rapid screening of chitinase producing strains by solid plate color reaction. At the same time, it screens the high-yield strain which producing chitinase through liquid fermentation. The results showed that it screened a Bacillus strain of high yield of producing chitinase, strain 2-3 produced the enzyme activity as high as 284U/ml. And we have researched on some enzymatic characteristics of chitinase. In accordance with the experimental determination of enzyme activity, the optimum substrate of chitinase was colloidal chitin, the optimum pH was 6.5 and the optimum temperature was 60℃,higher temperature was promoting the hydrolysis reaction of chitin. In addition, by salting-out experiments, the best salting-out concentration of chitinase was 70% of (NH4)2SO4 saturation.
Keywords：Chitin;Chitinase; Enzyme activity; Enzymatic characteristics
