Abstract：According to the nature of Monascus, the paper collected by sterile filtration of red yeast rice milled in Monascus spore suspension, using plate dilution method, realized from the simple separation of red yeast rice Monascus. According to the biological characteristics of Monascus, Summed up the principle of Monascus separation and separation media design and production, separation and purification method and process technology. Culture results showed that: Monascus is an acid, heat, alcohol-resistant fungus. After purification, the medium of Monascus the colonies were round, under cultivation at 30 ℃, 48h after the growth of the early colonies were white, and then into a light pink, red, direct purple, dark red, but the edge of the colony white. The colonies were cultured for 12 days and the colonies were dark red. Microscopic examination observed mycelium, mycelium with diaphragm, multi-core, branches very complex, spores arranged in chains, conidia are spherical cleistothesium top. After hours of Monascus pure, liquid medium for the fermentation of different carbon sources, nitrogen sources, pH were the single factors and five levels of testing, to determine the level of the three good, and then for three factors and three level orthogonal test to determine the production of Monascus red pigment in the best conditions. In the single-factor test, the carbon source for the corn flour, starch, malt extract powder are better; nitrogen as nitrate, soybean powder, peptone are better; pH to 3.2,4.0, 4.8 are better. Through three levels of the three-factor orthogonal experiment to determine the production of Monascus red
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pigment of the best medium for A1B2C2, is corn flour, sodium nitrate, pH4.0 was the best combination.
Key words: Red rice; Monascus; separation; purification; plate dilution method; red pigment; color value; liquid fermentation conditions.
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目 录
中文摘要 Ⅰ
英文摘要 Ⅲ
目录 Ⅴ
1. 绪论 1
1.1 红曲及红曲霉简介 1
1.2红曲霉分离原理的研究及培养条件的设计 2
1.3红曲霉的生物学特征 2
2. 实验部分 3
2.1主要实验仪器、设备 3
2.2主要实验试剂 3
2.3培养基 3
2.4实验方法与步骤 4
2.5红曲霉液体培养条件与产色素关系的研究 5
3. 结果与讨论 6
3.1红曲霉的分离纯化结果与分析 6
3.2红曲霉液体培养条件的单因素实验结果与分析 9
3.3正交试验最佳发酵培养条件的结果与分析 11
4．总结与展望 12
4.1总结 12
4.2展望 13
致谢 15
参考文献 16
