粗毛栓菌漆酶的分离与纯化
摘要:将粗毛栓菌接种在含有麦草粉的培养基中培养25d,过滤得粗酶液,尔后经超滤、盐析、离心、透析、浓缩,将透析液上样于Q-Sepharose F. F.层析柱,获得具有漆酶活性的组分,并测定上柱前、后漆酶的酶活力,以及溶液中的蛋白含量,从而测定漆酶的回收率和纯化度。
关键词: 粗毛栓菌;漆酶;离子交换层析
Sepration and Purification of Laccases in Trametes gallica
Abstract: Trametes gallica was cultivated in wheat powder for 25 days. The crude enzyme was ultrafiltrated, salted out , centrifugaled, dialyzed and concentration was applied to Q-Sepharose F.F. ion exchange column. The effluent with laccase acticity was obtained, Determine the column before and after the laccase enzyme activity and protein content of the solution to determine the recovery rate and purification degree of laccase.
Key words: Trametes gallica; laccase; ion exchange chromatography