粗毛栓菌漆酶的初步分离与纯化
摘要 将粗毛栓菌接种在含有麦草粉的培养基中培养25d,浸提得粗酶液,尔后经超滤浓缩、盐析和透析,将透析液上样于Q-Sepharose F. F.层析柱,获得具有漆酶活性的组分。
关键词 粗毛栓菌,漆酶,离子交换层析
Seperation and Purification of Laccases in Trametes gallica Fr.
Abstract Trametes gallica Fr. was cultivated in wheat straw powder for 25 days. The crude enzyme was ultrafiltrated, salted out, dialyzed and applied to Q-Sepharose FF ion exchage column. The effluent with laccase acticity was obtained.
Key words Trametes gallica, laccase, ion exchage chromatography