Abstract: The aim of this paper is to establish a high-performance liquid chromatography analysis techniques (HPLC) of the feed-methyl-testosterone levels. With 95 percent methanol-water solution to extract the feed-methyl-testosterone, some extracts from a neutral alumina Qingye-purification; HPLC system uses C18 column, a mobile phase of methanol: water (75:25 V / V), UV detection at 254 nm. The method of the range of 0.05 ~ 5.0 μ g / mL, linear regression equation y = 52.546x +0.7753, the correlation coefficient: r = 0.9998. To feed methyl-testosterone qualitative detection limit of 1 mg / kg, quantitative detection limit of 2 mg/Kg, when added to the concentration of 2 mg / Kg ~ 20 mg / Kg, the recovery rate was 86.5 percent to 108.8 percent. The method is simple and quick, from better-performing, is a feed-methyl-testosterone accurate detection of residues, and efficient analysis methods.
