Abstract: In this paper，the progress on determination of Sudan dyes by HPLC were reviewed. The contents include the pretreatment and purification of sample，the selection of extraction agent of Sudan dyes，wavelength，mobile phase，chromatographic detector，and so on. In the part of experiment， the content of SudanⅠ、SudanⅡ、SudanⅢ、SudanⅣ in chick egg yolk has been determined with a high performance liquid chromatographic method. These Sudan dyes were extracted with acetonitrile. The Sudan dyes were separated on a ZorboxSB-C18 column(4.6mm×150mm) with a mixture of acetonitrile、acetone、formic acid and water in certain varied ratio as mobile phase. They were then detected at two shifted wavelength 478nm-520nm，with it the experiment gains good effect for separation and low detecting limit. The recoveries of SudanⅠ、SudanⅡ、SudanⅢ、SudanⅣ were 85%、90%、92%、95% respectively. The relative standard deviations were 2.7%、4.3%、5.3%、3.5% respectively. The method is simple, rapid and accurate，and can meet the needs of practical determination entirely.
