Abstract: Chloramphenicol (Chloramphenicol, CAP) is an effective broad-spectrum antibiotics, but also on human existence of serious side effects. In this paper, solid-phase extraction (SPE) pre-treatment purification technology, extracted with ethyl acetate, n-hexane skim, C18 column purification, high-performance liquid chromatography - tandem mass spectrometry (LC-MS/MS) Determination of fish Chloramphenicol residues in, the standard method used to chloramphenicol peak area / chloramphenicol-D5 area of quantitative, 321.0/256.9 as a quantitative-right. Chloramphenicol linear range of detection are 0.05 μ g / kg ~ 1.2 μ g / kg, recovery rate of 70% to 120%, 321.0/256.9 standards of the lowest correlation coefficient of 0.99. Usage of MS have high sensitivity and stability, is recommended by the FDA confirmed methods, the use of quality will become the preferred method confirmed.
Keywords：Chloramphenicol; liquid chromatography - tandem mass spectrometry; isotope internal standard quantitative
