Study on the Extraction Technology of Polysaccharide from Agaricus Blazei Murill
Abstract: According to the single factor experiment of the extraction from Agaricus Blazei Murill, five factors of Uniform Design as the concentration of ethanol; multi-enzyme quantity; enzymolysis temperature and time; particle size has been ascertained. The optimum technological condition was determined after cinic fitting, ethanol/water(75∶25,V/V); enzyme2.0%; temperature for 45℃; extract 2 times and 1.5h for each; 300 mesh ABM. The extraction percent of ABM is 44.17%, the content of polysaccharide is 42.3%. It's 1.69 fold more than hot water extraction, and compare with cellulase、papain or pectinase it's increase 25.84%、14.73% and 29.15% for fractionation. The result could be summarized as follows: the optimization of extracting polysaccharide from ABM with Uniform Design and Regression Analysis will get more polysaccharide extractive percent、polysaccharide content and polysaccharide yield than literature published anterior.