Cornel Several triterpenoid acid content by HPLC method
Abstract:Through this experiment HPLC method, the main Cornel Triterpenoid acid (ursolic acid and oleanolic acid) extract of the mobile phase experiment, by comparing the ratio of different solvents, different velocities, different pH conditions, basically decided on a better Isolation conditions, And in accordance with the established sample processing methods and separation conditions, Cornel ursolic acid and oleanolic acid content was determined, the results showed that: 95 percent ethanol extract of Cornel triterpenoid acid, methanol: water (pH=3) = 9:1 as mobile phase, measured Cornel samples oleanolic acid and ursolic acid content of the average for 25.25 ug / ml and 143.2 ug / ml.