Primary Research of Streptococcal Bacteriophage Lysin in Escherichia coi
Abstract：This article to investigate the optimal high-density fermentation of recombinant bacteria plyC induced in E. coli conditions. Firstly, make use of 5L autocontrol fermentor for streptococcal bacteriophage lysin chain A (plyCA) fed batch fermentation bacteria feeding on feeding time, induced time, induced dose of the streptococcus bacteriophage lyase (plyC) A chain and B chain ( plyCA, plyCB) expressed in E. coli to study the impact of the situation to SDS-PAGE detection method to detect protein expression. The results show that the feeding time after 3.5 h in the fermentation by adding, to a certain speed continuous feeding, and the addition of induction agent training. The other to 1.7 mmol/L IPTG induced expression experiments show that the induction time of 4 hours lyase of streptococcal bacteriophage lysin recombinant expression produced of the highest purpose. Induced with 30 μL IPTG to a final concentration of 1 mmoL/L may cause PlyCA and the PlyCB expression quantity is relatively high
Key words: PLYC; streptococcal bacteriophage lysin;. E.coil; high cell-density fermentation; Induction expression.
