蛋白制品中三聚氰胺及氰尿酸含量的检测方法研究

论文编号:SW079  论文字数:8436,页数:26

摘 要：建立了高效液相色谱法检测乳制品中三聚氰胺及氰尿酸的方法。样品由1%三氯乙酸-乙腈超声提取，加入蛋白沉淀剂进行纯化，滤液经0.45m膜后进样分析。三聚氰胺的色谱条件及结果：色谱柱ZORBAX SB-Aq（250mm×4.6mm，5m），流动相为磷酸-三乙胺溶液（pH=3），流速为0.6ml/min，检测波长为240nm，回收率为101.7～124.0%，相对标准偏差在3.5～3.9%。氰尿酸的色谱条件及结果：色谱柱ZORBAX SB-Aq（250mm×4.6mm，5m），流动相为磷酸-三乙胺溶液（pH=3），流速为0.6ml/min，检测波长为225nm。采用HPLC方法测定蛋白制品中三聚氰胺及氰尿酸的含量具有高效、快速、准确、样品浪费少等等优点。适合企业及检测部门使用。
关键词：三聚氰胺；氰尿酸；高效液相色谱（HPLC）；蛋白制品

Detection Method of Melamine and Cyanuric acid in the protein products

 
Abstract: Established a high-performance liquid chromatography detection of dairy products of melamine and cyanuric acid method. Samples were extracted by blending with 10% TCA and acetonitrile. The extract was purified by precipitating protein. Filtrated 0.45m membrane. The HPLC conditions of melamine: ZORBAX SB-Aq（250mm×4.6mm, 5m）, phosphoric acid-triethylamine as mobile phase and UV detection at 240nm. The average recoveries were 101.7∼124.0% and RSD was 3.5∼3.9%.The HPLC conditions of Cyanuric acid: ZORBAX SB-Aq（250mm×4.6mm, 5m）, phosphoric acid-triethylamine as mobile phase and UV detection at 225nm. HPLC method which was using in the determination of protein products of melamine and cyanuric acid ,it had advantage like high efficiency, rapid, accurate and so on. It is suitable for detection of enterprises and departments.
Keywords：melamine ;cyanuric acid ;High Performance Liquid Chromatography (HPLC); protein products

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
1.1  三聚氰胺及氰尿酸简介 1
1.2  三聚氰胺的毒性 2 
1.3  三聚氰胺的检测方法 3
1.4  高效液相色谱法简介 3
2.实验部分 4
2.1  实验材料 4
 2.2  样品处理 5
 2.3  溶液的配制 5
 2.4  实验方法 6
 2.5 标准曲线 6
 2.6 样品含量测定 8
3．结果与讨论 8
 3.1 三聚氰胺检测结果与讨论    8
 3.2 氰尿酸检测结果与讨论 17
4．总结与展望 20
致谢 21
参考文献 22