Abstract: Medicinal drug is a special kind of commodity, especially for chinese materia medica preparation , complex sentence , and most effective elements of traditional chinese medicine is not very clear ,its quality directly connected to people’s health and life. Therefore, it is of great significance to research on the qualification control of drug to ensure its safety and effectiveness. We have developed a quantitative method. HPLC was applied to determinate the two effective components in the chief herb-YC, with the liquid phase 0.025mol/L H3PO4(pH was adjusted to 3.0 with triethylamine)-methol-acetonitrile; the detection wavelength:345nm, the rate:1.0 ml/min and the column temperature:30℃. Results showed that the linear range of 6,7-dimedthoxycoumarin is 1.3-13ug/ml with the recovery of 96.62%; the linear range of 6-hydroxy-7-methoxycoumarin is 2.36-23.6ug/ml with the recovery of 96.50%. The analytical method is simple, sensitive and high reproducibility, thus provide qualitative and quantitative proof for the quality control of Capsule YBGY.
Keywords：YBGBcapsule; High Performance Liquid Chromatography(HPLC); quantitative analysis
